Variation in subgingival flora of abutments before and after removal of nickel-chromium alloy porcelain-fused-to-metal restoration

doi:10.3969/j.issn.2095-4344.2013.51.009

Abstract

Abstract:

BACKGROUND: Currently, there are few reports on the effect of nickel-chromium (Ni-Cr) alloy porcelain-fused-to-metal (PFM) restoration on subgingival flora of abutment.
OBJECTIVE: To investigate the effect of the Ni-Cr alloy PFM restoration on subgingival flora ratio of abutment.
METHODS: Nine patients (12 teeth) who suspected that Ni-Cr alloy PFM could affect their health and therefore came to hospital to ask for removal of the prosthesis were selected in this study. Their subgingival plaques of abutment were obtained before and 1 month, 3 months after the Ni-Cr alloy PFM restorations were removed, respectively, and the changes of subgingival flora were observed and analyzed by the method of denaturing gradient gel electrophoresis.
RESULTS AND CONCLUSION: The images of denaturing gradient gel electrophoresis in subgingival bacteria of experimental group had significant changes at 1 and 3 months after Ni-Cr alloy PFM restorations removed, furthermore, there were significant differences in the images of denaturing gradient gel electrophoresis at 1 and 3 months. In addition, the specific bands were selected from denaturing gradient gel electrophoresis image that appeared before Ni-Cr alloy PFM restorations removed and weakened or disappeared after the removal of restorations, then 16S rDNA sequence in the specific bands were analyzed. The results showed that the gene sequences of these bands were closest related to Eikenella corrodens, Campylobacter rectus and Eubacterium saphenu. These findings indicated that the Ni-Cr alloy PFM restorations would result in the changes of the proportion of subgingival microflora and increases in the detection rates of some periodontal pathogens.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, chromium alloys, metal ceramic alloys, periodontitis

CLC Number:

R318

Guo Da-wei, Song Ling, Zhang Chun-yan, Cao Yang, Li Jing-wen, Liang Xing. Variation in subgingival flora of abutments before and after removal of nickel-chromium alloy porcelain-fused-to-metal restoration[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(51): 8834-8840.

Figures/Tables 4

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